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Recombinant Proteins T4 Dna Ligase Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant insert  (New England Biolabs)
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recombinant human ubiquitin ligase e3  (Yeasen Biotechnology)
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. <t>Recombinant</t> purified PavDREB1E-His was used to detect potential <t>E3</t> <t>ubiquitin</t> ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.
Recombinant Human Ubiquitin Ligase E3, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. <t>Recombinant</t> purified PavDREB1E-His was used to detect potential <t>E3</t> <t>ubiquitin</t> ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.
Recombinant Mouse Muc5ac Protein, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant plasmids pctftt16  (TaKaRa)
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. <t>Recombinant</t> purified PavDREB1E-His was used to detect potential <t>E3</t> <t>ubiquitin</t> ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.
Recombinant Plasmids Pctftt16, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. <t>Recombinant</t> purified PavDREB1E-His was used to detect potential <t>E3</t> <t>ubiquitin</t> ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.
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recombinant human flap endonuclease 1  (New England Biolabs)
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Recombinant Human Flap Endonuclease 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. <t>Recombinant</t> purified PavDREB1E-His was used to detect potential <t>E3</t> <t>ubiquitin</t> ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.
Pcdh Mutant Genes Recombinant Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. Recombinant purified PavDREB1E-His was used to detect potential E3 ubiquitin ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.

Journal: Plant Physiology

Article Title: The PavDREB1E–PavD8–PavHY5 module integrates light and gibberellin signals to regulate sweet cherry anthocyanin biosynthesis

doi: 10.1093/plphys/kiaf616

Figure Lengend Snippet: PavCOP1-1 and PavCOP1-2 ubiquitinate PavDREB1E, PavD8, and PavHY5. A to C) PavDREB1E and PavCOP1-1 interact, as determined by LCI assay, Y2H assay, and GST pull-down assay. D) PavCOP1-1 ubiquitinates PavDREB1E in vitro. Recombinant purified PavDREB1E-His was used to detect potential E3 ubiquitin ligase activity in the presence of ATP, ubiquitin (Ub), an E1 Ub-activating enzyme, an E2 Ub-conjugating enzyme, and recombinant PavCOP1-1-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavDREB1E ubiquitination. E to G) PavD8 and PavCOP1-2 interact, as determined by LCI assay, Y2H assay, and His pull-down assay. H) PavCOP1-2 ubiquitinates PavD8 in vitro. Recombinant purified PavD8-His was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavD8 ubiquitination. I to K) PavHY5 and PavCOP1-1 or PavCOP1-2 interact, as determined by a Y2H assay and His pull-down assays. L) PavCOP1-1 and PavCOP1-2 ubiquitinate PavHY5 in vitro. Recombinant purified PavHY5-MBP was used to detect potential E3 Ub ligase activity in the presence of ATP, Ub, E1, E2, and recombinant PavCOP1-1-GST or PavCOP1-2-GST. Immunoblot analysis with an anti-Ub antibody was performed to detect PavHY5 ubiquitination.

Article Snippet: In vitro ubiquitination assays were performed using the Recombinant Human Ubiquitin Ligase E3 (YEASEN, Shanghai, China) kit, following the manufacturer's instruction.

Techniques: Y2H Assay, Pull Down Assay, In Vitro, Recombinant, Purification, Ubiquitin Proteomics, Activity Assay, Western Blot